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雙光子成像系統

网上买足球GENGXINSHIJIAN:2018-08-30

簡要描述:

网上买足球SHUANGGUANGZIXISHOU/JIFAJISHUDEYUANLI:ZAIQIANGGUANGJIFAXIA,FENZITONGSHIXISHOULIANGGEGUANGZI,CONGJITAIYUEQIANDAOLIANGBEIGUANGZINENGLIANGDEJIFATAIDEGUOCHENG。

       雙光子吸收/激發技術的原理:在強光激發下,分子同時吸收兩個光子,從基態躍遷到兩倍光子能量的激發態的過程。兩個光子可以是相同波長的,也可以是不同波長的,但必須是同時吸收(兩個光子到達被激發分子的時間間隔小于1飛秒)。可以這樣理解,先吸收一個光子的能量躍遷到一個虛擬的中間態,然后再吸收一個光子的能量躍遷到激發態。 雙光子顯微鏡相對于共聚焦等其他顯微鏡的優點: ~ 光損傷小:由于雙光子顯微鏡使用的是可見光或近紅外光作為激發光源,這一波段的光對活體細胞和組織的光損傷小,適用于長時間的研究; ~ 穿透能力強:相對于紫外光,可見光和近紅外光都具有更強的穿透能力,因而受生物組織散射的影響更小,解決對生物組織中深層物質的層析成像研究問題;研究表明,共聚焦熒光成像的成像深度一般在50微米左右,而雙光子顯微鏡的成像深度可達1000微米; ~ 高分辨率:由于雙光子吸收截面很小,只有在焦平面很小的區域內可以激發出熒光,雙光子吸收僅局限于焦點處的體積約為波長3次方的范圍內

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